Helicobacter pylori is reportedly related to gastroduodenal damage, But, the mechanism for H, pylori induced gastric mucosal injury remains obscure,
H. pylori has high urease activity to produce ammonia from urea in stomach, In this study, we evaluated the cut-off value of gastric ammonia in basis of Giemsa stain and relationships of ammonia and urea nitorgen between in gastric juice and in
serum,
We also evaluated usefulness of diagnostic method of gastric ammonia test according to cut-off value in basis of Giemsa stain on chronic gastritis(n=5), And we compared gastric ammonia test with rapid urease test(CLO test),
@ES The results were as followed:
@EN 1, Thirty-one patients (62%) had H, pylori identifiable in Giemsa stain,
2. The ammonia concentration of gastric juice was significantly higher in H, pylori-infected gastritis than in H, pylori-negative gastritis(61,76±43,0 vs 20,96±17,77mmol/dl, p<0,001),
3. There were no statistical differences between ammonia and urea nitrogen in gastric juice and serum,
4. Cut-off value of gastric ammonia concentration according to Giemsa stain was 7,78mmol/dl(Ep sign test),
5. When we compared gastric ammonia test with rapid urease test(CLO test), the sensitivity of the methods were 100% vs 97%, and the specificity were 57% vs 95%, and probability were 79% vs 97%,
We concluded that the ammonia concentration of gastric juice is significantly higher in H pylori-infected gastritis than H pylori-negative gastritis and the gastric ammonia test in this study is useful in diagnosis of H, pylori infected
gastritis,
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